Protocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score

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dc.contributor.authorÖzdag, Hilal
dc.contributor.authorIDhttp://orcid.org/0000-0001-7940-2499tr_TR
dc.contributor.departmentBiyoteknoloji Enstitüsütr_TR
dc.contributor.otherBelder, Nevin
dc.contributor.otherTekin, Nilgün
dc.date.accessioned2020-04-29T07:14:39Z
dc.date.available2020-04-29T07:14:39Z
dc.date.issued2016
dc.description.abstractAs a part of an international study on the molecular analysis of Diffuse Large B-cell Lymphoma (DLBCL), a robust protocol for gene expression analysis from RNA extraction to qRT-PCR using Formalin Fixed Paraffin Embedded tissues was developed. Here a study was conducted to define a strategy to validate the previously reported 6-gene (LMO2, BCL6, FN1, CCND2, SCYA3 and BCL2) model as predictor of prognosis in DLBCL. To avoid variation, all samples were tested in a single centre and single platform. This study comprised 8 countries (Brazil, Chile, Hungary, India, Philippines, S. Korea, Thailand and Turkey). Using the Kaplan-Meier and log rank test on patients (n=162) and two mortality risk groups (with those above and below the mean representing high and low risk groups) confirmed that the 6-gene predictor score correlates significantly with overall survival (OS, p < 0.01) but not with event free survival (EFS, p=0.18). Adding the International Prognostic Index (IPI) shows that the 6-gene predictor score correlates significantly with high IPI scores for OS (p < 0.05), whereas those with low IPI scores show a trend not reaching significance (p=0.08). This study defined an effective and economical qRT-PCR strategy and validated the 6-gene score as a predictor of OS in an international setting.tr_TR
dc.description.indexwos
dc.description.indexScopus
dc.identifier.endpage83329tr_TR
dc.identifier.issue50tr_TR
dc.identifier.startpage83319tr_TR
dc.identifier.urihttps://doi.org/110.18632/oncotarget.13066tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/71196
dc.identifier.volume7tr_TR
dc.language.isoentr_TR
dc.relation.isversionof10.18632/oncotarget.13066tr_TR
dc.relation.journalOncotargettr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subject6-gene predictor scoretr_TR
dc.subjectDiffuse large B-cell lymphoma (DLBCL)tr_TR
dc.subjectFormalin fixed paraffin embedded tissue (FFPE)tr_TR
dc.titleProtocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor scoretr_TR
dc.typeArticletr_TR

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