In vitro Regeneration Studies of Vuralia turcica Using Unpollinated Ovaries

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dc.contributor.authorTekdal, Dilek
dc.contributor.departmentOthertr_TR
dc.contributor.facultyOthertr_TR
dc.date.accessioned2022-09-13T08:12:39Z
dc.date.available2022-09-13T08:12:39Z
dc.date.issued2022
dc.description.abstractVuralia turcica is an endemic plant species with a polycarpellary gynoecium and has the ability to survive in salt stress. However, the maintenance of this species is currently challenging because of climatic changes in the plant’s habitat, intensive agricultural purposes, grazing, and the like. Gynogenesis is a promising method for micropropagation and a useful technic for genetic engineering. The objective of this study was to analyze the gynogenesis of V. turcica. The experiments described here were implemented during the flowering time of V. turcica (spring) using unpollinated ovaries. Modified MS and B5 induction media and different plant growth regulators (BAP, GA3, 2,4-D, and KIN) were compared. The comparison of both media (MS and B5) showed that MS medium supplemented with 2,4-D and KIN was the best medium for in vitro unfertilized ovary culture and gave the best result of regenerated plants per 30 ovaries, and 92% callus was obtained. Only the control groups showed very low scores or no plants. From plant regeneration and subcultures, a total of 60 plants were produced, all green. These results are of interest to the in vitro conservation of endangered plant species like V. turcica.tr_TR
dc.description.indexWostr_TR
dc.description.indexScopustr_TR
dc.identifier.endpage120tr_TR
dc.identifier.issn/e-issn2148-9297
dc.identifier.issue01tr_TR
dc.identifier.startpage115tr_TR
dc.identifier.urihttps://doi.org/10.15832/ankutbd.778783tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/83969
dc.identifier.volume28tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.15832/ankutbd.778783tr_TR
dc.relation.journalTarım Bilimleri Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Başka Kurum Yazarıtr_TR
dc.subjectlegumestr_TR
dc.subjectB5tr_TR
dc.subjectMStr_TR
dc.titleIn vitro Regeneration Studies of Vuralia turcica Using Unpollinated Ovariestr_TR
dc.typeArticletr_TR

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Cilt:28 Sayı:01 (2022)